بررسی فاکتورهای بیماری‌زایی سفیدک پودری جو در استان فارس

دو فصلنامه تحقیقات بیماریهای گیاهی

مقاله 2، دوره 1، 3(پاییز)، مهر و آبان 1392، صفحه 17-25   

نوع مقاله: مقاله پژوهشی

نویسندگان

محمد جواد مرمت¹؛ سیدمحمدرضا موسوی^{* 2}؛ منصوره کشاورزی³؛ محمد تائب⁴

¹کارشناس ارشد دانشگاه آزاد اسلامی، واحد علوم و تحقیقات تهران، ایران.

²دانشگاه آزاد اسلامی واحد مرودشت، گروه بیماری شناسی گیاهی، مرودشت، ایران.

³موسسه تحقیقات اصلاح نهال و بذر، کرج، ایران.

⁴دانشگاه آزاد اسلامی واحد علوم و تحقیقات تهران، ایران.

تاریخ دریافت: 13 آبان 1392،  تاریخ پذیرش: 13 آبان 1392 

چکیده

اولین گام در جهت تشخیص و تهیه ارقام مقاوم به بیماری سفیدک پودری جو، شناسایی فاکتورهای بیماریزایی جمعیت عامل آن است. بدین منظور در تحقیقی که در سال 1387 در استان فارس صورت گرفت تعداد ده جدایه از مناطق مختلف استان جمع آوری و روی یک رقم حساس جو تکثیر شدند. سپس جداگانه روی گیاهچه تعداد 19 رقم تقریباً ایزوژنیک که رقم پایه آنها پالاس و دارای ژنهای مقاومت متفاوتی بودند و یک رقم حساس مایه زنی شدند. واکنش لاین ها از نظر دوره کمون، تیپ و شدت آلودگی یادداشت برداری شد. نتایج نشان داد که فاکتورهای بیماریزایی در استان فارس فراوانی متغیری دارند . بیماریزایی برای ژن های مقاومتMl-a 3 و  Ml-a23 دارای بیشترین فراوانی در مناطق مورد اجرای آزمایش بوده وپس از آن بیشترین فراوانی بیماری زایی به ترتیب برای ژ ن های، Ml-K ،Ml-a7+Ml(No3)،Ml-hوMl-a12+Ml(Em2) ثبت گردید. کمترین فراوانی بیماری زایی برای ژن های مقاومتMl-a 22 وMl-a6+Ml- a14 دیده شد برای ژنهای Ml-(La)،Ml-a13+Ml-(Ru3) ،Ml-a10+Ml-(DU2) ،Ml-a9 ،Ml-al+Ml(a12) ،Ml-a8 ،Ml-05 ،Ml--Ml-(cp) در هیچ یک از مناطق بیماری زایی دیده نشد Ml-at و Ml-p ،Ml- K(1). دوره کمون تمام جدایه ها تقریباً از یک روند زمانی خاص پیروی می کرد.

کلیدواژه‌ها

جو؛ سفیدک پودری؛ لاین های تقریباً ایزوژنیک؛ فاکتورهای بیمار یزایی

عنوان مقاله [English]

Investigation on barely powdery mildew virulence factors in Fars province,Iran

نویسندگان [English]

M.J. Maramat¹؛ mohammad reza Moosavi²؛ M. Keshavarzi³؛ M. Taeb⁴

چکیده [English]

 
Determining the pathogenicity factors of barely powdery mildew casual agent is the first step in identifying and producing resistant cultivars. During growing season 2008, ten isolates of barely powdery mildew were collected from different parts of Fars province (Iran). Mass propagated on a susceptible barely cultivar. Seedlings of 19 near-isogenic barley lines, obtained from Palas base type and containing various resistance genes, were inoculated with the collected isolates. A susceptible cultivar (cv. Afzal) was used as positive control. Later on,reactions of lines were recorded according to latent period, type and severity of disease. The detected pathogenicity factors in Fars province seemed to have variable frequencies.Virulence factors against Ml-a3 and Ml-a23 resistance genes were the most abundant in the tested area, while for Ml-K, Ml-a7+Ml-(NO3), Ml-h and Ml-a12+Ml (Em2) genes were the second most abundant. For the Ml-a22 and Ml-a6+Ml-a14 the least frequency of pathogenic potential was observed. There are no pathogenicity factors against Ml-05, Ml-g-Ml-(Cp), Ml-(La),Ml-a8, (Ml-a1, Ml(A12)), Ml-a9, Ml-a10+Ml-(DU2), Ml-a13+Ml-(Ru3), Ml-K(1), Ml-P and Ml-at resistance genes in Fars province. Almost all barley lines followed a similar pattern in their latent period.

کلیدواژه‌ها [English]

Barely, powdery mildew, near-isogenic lines, pathogenicity factors

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