Histopathological and Stereological Study of the Effects of Gallic Acid Administration on Hippocampal Neuronal Density after Trimethyltin Toxication

Report of Health Care

مقاله 4، دوره 4، شماره 2، شهریور 2018، صفحه 22-27   

نوع مقاله: Original Article

نویسندگان

heydar aqababa^{* 1}؛ Mona Ghezelbash²؛ Mohamad Amin Edalatmanesh²؛ Shirin Dehghan¹

¹Department of Biology, Arsanjan Branch, Islamic Azad University, Arsanjan, Iran

²Department of Biology, Shiraz Branch, Islamic Azad University, Shiraz, Iran

تاریخ دریافت: 16 بهمن 1396،  تاریخ بازنگری: 31 فروردین 1397،  تاریخ پذیرش: 18 اردیبهشت 1397 

چکیده

Introduction: Trimethyltin is a methylated organotin, which induces selective damage and neuronal death in human and rodents’ CNS. On the other hand, the neuroprotective effects of Gallic acid can prevent toxicity of trimethyltin. In the current study, the repairing effect of Gallic acid in cell injury caused by trimethyltin in rats was evaluated. Also, this study assessed the effects of Gallic acid on neuronal density of the different hippocampal areas of intoxicated rats with trimethyltin. Methods: 30 rats were divided in three groups: the no treatment control group, the control group, and the experimental group with intraperitoneal injection of trimethyltin (8 mg/kg) and Gallic acid solvent for 14 days. In the experimental group, after the administration of trimethyltin, 50 mg/kg of Gallic acid was injected. It was continued for 14 days. Finally, the rats were killed with transcardial perfusion. Histopathological and stereological analysis was performed on the rats. In this research, Kolmogorov-Smirnov test, one-way analysis of variance, and Tukey’s post-hoc test were used. Results: The results of this study showed that neuronal density of different hippocampal areas of the no treatment control group was significantly increased compared to the rats of the control and experimental groups (P<0.05), while chronic administration of Gallic acid could prevent apoptosis and protect hippocampal cells. Conclusions: According to the results, it is suggested that chronic administration of Gallic acid decreases the effects of trimethyltin and therefore, it prevents the reduction of hippocampal cells.

کلیدواژه‌ها

Hippocampus؛ Gallic Acid؛ Trimethyltin؛ Rat

مراجع

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