ارزیابی حضورباکتری های کمپیلوباکترججونی و لیستریا مونوسیتوژنزدرمنابع آب شرب شهرکرمانشاه قبل از کلرزنی براساس مقایسه کشت وPCR

فصلنامه علمی مهندسی منابع آب

مقاله 9، دوره 16، شماره 57، مرداد 1402، صفحه 121-130   

نوع مقاله: مقاله پژوهشی

شناسه دیجیتال (DOI): 10.30495/wej.2023.5825

نویسندگان

سهیلا حیدریان¹؛ آتوسا فردوسی^{* 2}؛ محمدحسن شاه حسینی³؛ طاهر محمدیان⁴

¹Ph.D. Student of Microbiology, shahr-e- Qods Branch, Islamic Azad University, Tehran, Iran

²Supervisor, Ph.D. of mycology, shahr-e- Qods Branch, Islamic Azad University, Tehran, Iran

³Supervisor, Ph.D. of Biotechnology, shahr-e- Qods Branch, Islamic Azad University, Tehran, Iran

⁴Advisor, Ph.D. of Biochemistry, shahr-e- Qods Branch, Islamic Azad University, Tehran, Iran

تاریخ دریافت: 04 فروردین 1400،  تاریخ بازنگری: 12 بهمن 1400،  تاریخ پذیرش: 17 بهمن 1400 

چکیده

زمینه وهدف : در نتیجه رشد روزافزون جمعیت جهان پیش بینی می شود که آب به عنوان یکی از کمیاب ترین منابع در قرن بیست و یکم مطرح باشد .با وجود پیشرفت های فراوان در زمینه تصفیه آب و فاضلاب ،بیماری های ناشی از آب هنوز یک تهدید برای سلامت مردم دنیا محسوب می شود.

باکتری لیستریا مونوسیتوژنز یک پاتوژن است که باعث لیستریوزیس میگردد. همچنین این پاتوژن می‌تواند باعث مننژیت، سپتی سمی و وقوع سقط جنین در انسان شود. یکی از راه های انتقال این میکروارگانسیم آب ومواد غذایی است. شناسایی سریع و دقیق آن در پیشگیری از موارد عفونت نقش بسزایی دارد.

وهمچنین با توجه به اهمیت کمپیلوباکتر ججونی درآب و صنایع غذایی و ایجاد عفونت و مسمومیت و مشکلات گوارشی در انسان، شناسایی این باکتری‌ می‌تواند گامی مؤثر در جلوگیری از آلودگی آب به کمپیلوباکتر‌ججونی باشد.

هدف ازاین مطالعه شناسایی باکتری های لیستریا مونوسیتوژنز وکمپیلوباکترججونی باروش کشت وPCR ومقایسه دوروش درمنابع تامین کننده آب شهر کرمانشاه میباشد.

روش کار: نمونه ها شامل1چشمه 1سراب و1سدو124حلقه چاه عمیق از منابع تامین کننده آب شهر کرمانشاه که در 18مخزن ذخیره جمع آوری شد ه استخراج DNA از باکتری استاندارد کمپیلوباکتر ججونی وهمچنین لیستریا مونوسیتوژنزبا استفاده از کیت DNG-Plus انجام گرفت واکنش زنجیره ایی پلیمراز با استفاده از آغازگرهای اختصاصی بهینه شد. پس از تعیین اختصاصیت و حد تشخیص واکنش زنجیره ایی پلیمراز نمونه های جمع آوری شده آب مورد برسی قرارگرفت وهمزمان هم نمونه ها کشت داده شد.ومورد برسی قرار گرفت.

کلیدواژه‌ها

: لیستریا مونوسیتوژنز؛ کمپیلوباکترججونی؛ واکنش زنجیره ایی پلیمراز؛ آب شرب

عنوان مقاله [English]

Evaluation of the presence of Campylobacter Jejuni and Listeria Monocytogenes in water sources of Kermanshah city before chlorination based on comparison of culture and PCR

نویسندگان [English]

Soheila Haydarian¹؛ Atousa Ferdousi²؛ Mohammad-Hassan Shahhosseiny³؛ Taher Mohammadian⁴

¹Ph.D. Student of Microbiology, shahr-e- Qods Branch, Islamic Azad University, Tehran, Iran

²Supervisor, Ph.D. of mycology, shahr-e- Qods Branch, Islamic Azad University, Tehran, Iran

³Supervisor, Ph.D. of Biotechnology, shahr-e- Qods Branch, Islamic Azad University, Tehran, Iran

⁴4 Advisor, Ph.D. of Biochemistry, shahr-e- Qods Branch, Islamic Azad University, Tehran, Iran

چکیده [English]

Abstract: 
Introduction: As the world population grows, it is predicted that freshwater supplies will be rare in the 21st century. Despite abundant advances in water and wastewater treatment, waterborne diseases still threaten the health of the people of the world. Listeria monocytogenes bacterium is a pathogen that causes listeriosis. This pathogen can also cause meningitis, poisonous sepsis, and abortion in humans. One way of transmitting this microorganism is water and foodstuffs. Quick and accurate identification plays an important role in preventing infections. Also, due to the importance of Campylobacter jejuni in water and food industries and causing infection, toxication, and digestive problems in humans, the identification of this bacterium can be an effective step in preventing water contamination with Campylobacter jejuni. The aim of the present study was to identify Listeria monocytogenes and Campylobacter jejuni through culture and PCR and compare them in the water supply of Kermanshah city.
Methods: 18 samples were collected from different water supplies of Kermanshah. DNA was extracted from standard Campylobacter jejuni and Listeria monocytogenes using a DNG-Plus kit. PCR reaction was optimized using specific primers. After determining the specificity and PCR detection limit, the collected water samples were examined and at the same time, the samples were cultured and examined.
Findings: From 18 samples of water supply sources in Kermanshah by PCR, Campylobacter jejuni was isolated from all samples, and Listeria monocytogenes was isolated from 17 samples, and also 4 cases of Campylobacter jejuni and 2 cases of Listeria monocytogenes were isolated by culture method. The results showed that PCR has a better performance than culture for detecting Listeria monocytogenes and Campylobacter jejuni.

کلیدواژه‌ها [English]

Listeria monocytogenes, Campylobacter jejuni, PCR, drinking water

مراجع

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