اثر ضد قارچی عصاره‌های گیاهی بر قارچ عامل پژمردگی فوزاریومی توتون

دو فصلنامه تحقیقات بیماریهای گیاهی

مقاله 5، دوره 3، شماره 1، خرداد و تیر 1394، صفحه 47-62   

نوع مقاله: مقاله پژوهشی

نویسندگان

سید افشین سجادی^{* 1}؛ هدی عاصمی²

¹مربی، بخش گیاهپزشکی، مرکز تحقیقات و آموزش تیرتاش، بهشهر، ایران

²استادیار، بخش گیاهپزشکی، مرکز تحقیقات و آموزش تیرتاش، بهشهر، ایران.

تاریخ دریافت: 25 اسفند 1393،  تاریخ پذیرش: 25 اسفند 1393 

چکیده

بیماری پژمردگی فوزاریومی توتون، از بیماری­های عمده توتون در دنیا است که می­تواند خسارات زیادی را موجب شود. مهاراین بیماری باعصاره‌های گیاهی یکی از روش­های امن و دوستدار محیط زیست به شمار می­رود. هدف از این تحقیق تعیین فعالیت ضد قارچی عصاره‌ چند گیاه بر قارچ عامل پژمردگی فوزاریومی توتون (Fusarium oxysporum f.sp. nicotianae) تهیه شده با حلال­های مختلف بود. در این تحقیق عصاره‌های نه گونه گیاهی شامل نعناع گربه‌ای، توتون، آویشن کوهی، رازیانه، زوفا، بادرنجبویه پُرپر، بادرنجبویه، پونه کوهی ومریم گلیبا با استفاده از حلال‌های آب، استون، هگزان، اتانول و متانول با روش خیساندن استخراج و فعالیت ضد قارچی آنها در شرایط آزمایشگاه روی قارچ‌ عامل بیماری بررسی شد. آزمایش به صورت فاکتوریل در قالب طرح کاملاً تصادفی با سه عامل گیاه، حلال و غلظت و پنج تکرار انجام شد. بررسی حداقل غلظت بازدارندگی عصاره‌های گیاهی با استفاده از روش اختلاط با محیط کشت انجام شد. گیاهان نعناع گربه‌ای، توتون، آویشن کوهی، رازیانه، زوفا و بادرنجبویه پُرپر اثر بازدارندگی قابل توجهی بر رشد میسلیومی قارچ مورد بررسی در این مطالعه داشتند. بیشترین تاثیر بازدارندگی مربوط به عصاره استخراجی با حلال متانول بود. بهترین غلظت بازدارندگی مربوط به غلظت 2000 قسمت در میلیون بود. حداقل غلظت بازدارندگی عصاره متانولی توتون، نعناع گربه‌ای، آویشن کوهی، رازیانه، بادرنجبویه پُرپر و زوفا بر قارچ‌ بیماری‌زای مورد بررسی 5/1، 5/1، 2، 3، 3 و 3 میلی­گرم بر میلی­لیتر بود. نتایج این تحقیق نشان از پتانسیل بالای عصاره متانولی توتون، نعناع گربه‌ای و آویشن کوهی در مهار زیستی قارچ عامل پژمردگی فوزاریومی توتون دارد.

کلیدواژه‌ها

مهار زیستی؛ ترکیبات طبیعی گیاهی؛ Fusarium oxysporum f.sp. nicotianae

عنوان مقاله [English]

Study of antifungal activity of plant extracts on tobacco Fusarium wilt agent

نویسندگان [English]

A. Sajjadi¹؛ H. Assemi²

چکیده [English]

Tobacco Fusarium wilt is one of the most important tobacco diseases in the world being able to impose heavy losses. The disease control using plant extracts was considered as a safe and green method. The purpose of current research was to determine the antifungal activity of some plant extracts against Fusarium oxysporum f.sp. nicotianae prepared with different solvents. In this study, crude extracts of nine plant species including catmint (Nepeta cataria), tobacco (Nicotiana tabacum), thyme (Thymus pubescens), fennel (Foeniculum vulgar), hyssop (Hyssopus officinalis), Badrashbi (Dracocephalum kotschyi), Balm (Melissa angustifolious), Nepeta (Mentha pulegium) and Salvia (Salvia verticilata) was extracted by maceration method using water, acetone, hexane, ethanol, and methanol as solvents. These extracts were tested in vitro for antifungal activity against tobacco Fusarium wilt. The experiment was performed in factorial arrangement based on completely randomized design with three factors of plant, solvent and concentration and five replications. The minimum inhibitory concentration of each extract was determined by medium mixing method. The crude extracts of tobacco, catmint, thyme, fennel, hyssop and badrashbi had remarkable inhibitory effect on mycelial growth of fungus. The maximum inhibitory effect was observed in methanolic extracts. The most inhibitory concentration was 2000 ppm. The minimum inhibitory concentration of methanol extract of tobacco, catmint, thyme, fennel, badrashbi, and hyssop the fungus was 1.5, 1.5, 2, 3, 3, and 2.5 mg/ml, respectively. The results showed the high potential of the methanol extract of tobacco, catmint and thyme which could be used as a biological product against tobacco Fusarium wilt.

کلیدواژه‌ها [English]

natural herbal products, biocontrol, Fusarium oxysporum f.sp. nicotianae

مراجع

1. Abad LR, DۥUrzo MP, Liu D, Narasimban ML, Reuveni, M, Zhu JK, Niu, X, Singh NK, Hasegawa PM and Bressan RA. 1996. Antifungal activity of tobacco osmotin has specificity and involves plasma membrane permeabilization. Plant Science 118:11–23.
2. Abdolmaleki M, Salari M, Bahraminejad S, Abbasi S and Panjehkeh N. 2008. Antifungal activity of cinnamon (Cinnamum zelanicum) crude extracts against some phytopathologenic fungi. Iranian Journal of Plant Pathology 44 (3): 255–261.
3. Abdolmaleki M, Bahraminejad S, Salari M, Abbasi S and Panjehkeh N. 2011. Study of antifungal effect Mentha piperita L. on plant pathogen fungi. Medical Plants 38: 26–34.
4. Abdolmaleki M, Bahraminejad S and Abbasi S. 2011. Study of antifungal effects of some of plant extracts for four plant pathogen fungi. Medical Plants 38: 148–155.
5. Al-Askar AA and Rashad YM. 2010. Efficacy of some plant extracts against Rhizoctonia solani on Pea. Journal of Plant Protection Research 50(3): 239–243.
6. Adams RP. 1995. Identification of essential oil components by gas chromatography/ mass spectroscopy. Allured Publishing. Carol Stream, 404p.
7. Alam A, Tripathi A, Vats S, Behera KK and Sharma V. 2011. In vitro antifungal efficacies of aqueous extract of Dumortiera hirsuta (Swaegr.) Nees against sporulation and growth of postharvest phytopathogenic fungi. Archive for Bryology 103: 1–9.
8. Al-Rahmah N, Mostafa A and Abdel-Megeed A. 2011. Antifungal and antiaflatoxigenic activities of some plant extracts. African Journal of Microbiology Research 5(11):1342–1348.
9. Amadioha, AC. 2000. Controlling rice blast in vitro and in vivo with extracts of Azadirachta indica. Crop Protection 19(1): 287–290.
10. Anonymous. 2012. Statistical repertoire of Iranian Tobacco Company. 52 p. (In Persian).
11. Aye SS and Matsumoto M. 2010. Effect of some plant extracts on Rhizoctonia spp. and Sclerotium hydrophilum. Journal of Medicinal Plants Research 5(16): 3751–3758.
12. Azadbakht M. 1999. Taxonomy of Medical Plants. Tehran: Teymorzadeh Publications Institute. 401 pp
13. Azimi AA, Delnavaz HB and Mansour GA. 2006. Antifungal effect of aqueous alcoholic and phenolic extracts of seed and leaves of Sorghum bicolour against Fusarium solani, Fusarium poa in Persian. Medical Plants 6(1): 26–32.
14. Bahraminejad S, Abbasi S and Fazlali M. 2011. In vitro antifungal activity of 63 Iranian plant species against three different plant pathogenic fungi. African Journal of Biotechnology 10(72): 16193–16201.
15. Burt S. 2004. Essential oils: their antimicrobial properties and potential applications in foods- a review. International Journal of Food Microbiology 94(1): 223–253.
16. Dissanayake MLMC and Kumari WKMT. 2012. Efficacy of various plant extracts to control Fusarium wilt of Polyscias balfouriana variety Marginata, Asian Journal Experimental Biological Science 3(1): 129–135.
17. Foroughi M, Mohammadi S and Ghasemi A. 2013. Antifungal activity of five medical herbs on the plant pathogenic fungus Rhizoctonia solani. Journal of microbial World 5(3): 115–121.
18. Gupta SK and Tripathi SC. 2011. Fungitoxic activity of Solanum torvum against Fusarium sacchari. Plant Protection Science 47(3): 83–91.
19. Hasanzadeh N. 2005.Technological implication of natural products in plant diseases management with special emphasis on fire blight. Agriculture Science 1: 53–68.
20. Huang JW. 1994. Control of Chinese leek rust with a plant nutrient formulation. Plant Pathology 3(1): 9–17.
21. Joseph B, Ahmad Dar M and Kumar V. 2008. Bioefficacy of plant extracts to control Fusarium solani f.sp. melongenae incident of Brinjal Wilt. Global Journal of Biotechnology 3(2): 56–59.
22. Lee SO, Choi GJ, Jang KS, Lim HK, Cho KY and Kim JC. 2007. Antifungal activity of five plant essential oils as fumigant against postharvest and soilborne plant pathogenic fungi. Plant Pathology Journal 23(2): 97–102.
23. Lucas GB. 1975. Disease of Tobacco, 3^rd edition, Biological consulting Associates, Releight, North Carolina. 621 p.
24. Mahboubi M, Feizabadi MM, Haghi K and Hossini H. 2007. Antimicrobial activity and chemical metabolites' of essential oil from Oliveria decumbens Vent. Iranian Journal of Medical and Aromatic Plants 24(1): 56–65 (In Persian).
25. Obongoya BO, Wagai SO and Odhiambo G. 2010. Phytotoxic effect of selected crude plant extracts on soil-borne fungi of common bean. African crop science Journal 18(1): 15–22.
26. Pirajno G, Scarito G and Salamone A. 2004. Fungistatic activity of essential oils of Laurus nobilis, Mentha piperita, and Ruta graveolens against Rhizoctonia solani Kuhn and Fusarium oxysporum (L) De Bary. Journal of Plant Pathology 84(4): 329–337.
27. Plotto A, Roberts D and Roberts R G. 2003. Evaluation of plant essential oils as natural postharvest disease control of Tomato (Lycopersicon esculentum). Acta Horticulturae 628: 737–745.
28. Sajjadi A and Assemi H. 2012. Evaluation of Trichoderma isolates for biocontrol of tobacco collar Sclerotinia rot in Golestan province. Iranian Journal of Applied plant protection 1(2): 73–84.
29. Sajjadi A, Hosseininejad A and Assemi H. 2012. Determination of damage of root knot nematode (Meloidogyne incognita) on some of tobacco commercial cultivar. Iranian Journal of Plant Pathology 80 (1): 13–22.
30. Sajjadi A and Assemi H. 2012. Identification of pathogenic soil-borne fungi of tobacco in Golestan province fields. Applied Plant Protection 1(3): 233–248.
31. Sajjadi A, Assemi H, Najafi M R and Moradi G. 2014. Study of effect some of plant extrctes on tobacco soilborne pathogen fungi. Annual Report, Tirtash Research and Education Center. 149–170.
32. Sehajpal A, Arora S and Kaur P. 2009. Evaluation of plant extracts against Rhizoctonia solani causing sheath blight of rice. The Journal of Plant Protection Sciences 1(1): 25–30.
33. Shahat A, Ibrahim A Y, Hendawy S F, Omer E A, Hammouda F M, Abdel-Rahman F H and Saleh M A. 2011. Chemical composition, Antimicrobial and Antioxidant activities of essential oil from organically cultivated fennel cultivars. Molecules, 16(1): 1366–1377.
34. Shariff N, Sudarshana M S, Umesha S and Hariprasad P. 2006. Antimicrobial activity of Rauvolfia tetraphylla and Physalis minima leaf and callus extracts. African Journal of Biotechnology 5(10): 946–950.
35. Suleiman MN. 2011. Antifungal properties of leaf extract of neem and tobacco on three fungal pathogens of tomato (Lycopersicon esculentum Mill). Advances in Applied Science Research 2 (4): 217–220.
36. Yanar Y, Gokce A, Kadioglu I, Cam H and Whalon, M. 2011. In vitro antifungal evaluation of various plant extracts against early blight disease (Alternaria solani) of potato. African Journal of Biotechnology 10(42): 8291–8295.
37. Yahya-abadi S, Zeabanejad E and Doudi M. 2011. Effect of plant extracts on growth of Aspergillus fungi. Journal of Herbal Drugs 2(1): 69–81.